10,894 research outputs found
Spatially resolved photo ionization of ultracold atoms on an atom chip
We report on photo ionization of ultracold magnetically trapped Rb atoms on
an atom chip. The atoms are trapped at 5 K in a strongly anisotropic
trap. Through a hole in the chip with a diameter of 150 m two laser beams
are focussed onto a fraction of the atomic cloud. A first laser beam with a
wavelength of 778 nm excites the atoms via a two photon transition to the 5D
level. With a fiber laser at 1080 nm the excited atoms are photo ionized.
Ionization leads to depletion of the atomic density distribution observed by
absorption imaging. The resonant ionization spectrum is reported. The setup
used in this experiment is not only suitable to investigate BEC ion mixtures
but also single atom detection on an atom chip
The cytolytic T lymphocyte response to the murine cytomegalovirus
Limiting dilution (LD) analysis with two modifications, the expansion and the restimulation LD assay, led to the detection and quantification of two distinct in vivo maturation stages within the lineage of virus- specific self-restricted CTL after infection of mice with the murine cytomegalovirus (MCMV). A low frequency set, representing an average of 15% of the specifically activated CTL-P in a draining lymph node, generated virus-specific lytic activity in the absence of antigen, solely under expansion conditions provided by growth and differentiation interleukins. These cells were considered to be active and were denoted antigen-independent or interleukin-receptive CTL-P (IL- CTL-P). A high frequency set required additional antigen in vitro to generate functionally active clones, and therefore the cells were termed antigen-dependent. Both sets are present in vivo simultaneously at the peak of the acute immune response and represent antigen- activated cells because their existence strictly depends on a preceding priming event. IL-CTL-P disappear quickly after acute infection and are absent during the memory state. It is proposed that the isolation of IL- CTL-P could serve to detect viral antigen expression during persistent and/or recurrent herpes virus infections
Best practices for HPM-assisted performance engineering on modern multicore processors
Many tools and libraries employ hardware performance monitoring (HPM) on
modern processors, and using this data for performance assessment and as a
starting point for code optimizations is very popular. However, such data is
only useful if it is interpreted with care, and if the right metrics are chosen
for the right purpose. We demonstrate the sensible use of hardware performance
counters in the context of a structured performance engineering approach for
applications in computational science. Typical performance patterns and their
respective metric signatures are defined, and some of them are illustrated
using case studies. Although these generic concepts do not depend on specific
tools or environments, we restrict ourselves to modern x86-based multicore
processors and use the likwid-perfctr tool under the Linux OS.Comment: 10 pages, 2 figure
Multidimensional perfect fluid cosmology with stable compactified internal dimensions
Multidimensional cosmological models in the presence of a bare cosmological
constant and a perfect fluid are investigated under dimensional reduction to
4-dimensional effective models. Stable compactification of the internal spaces
is achieved for a special class of perfect fluids. The external space behaves
in accordance with the standard Friedmann model. Necessary restrictions on the
parameters of the models are found to ensure dynamical behavior of the external
(our) universe in agreement with observations.Comment: 11 pages, Latex2e, uses IOP packages, submitted to Class.Quant.Gra
Multiple mRNA isoforms of the transcription activator protein CREB
We have characterized cDNA clones representing mouse
CREB (cyclic AMP responsive element binding protein)
mRNA isoforms. These include CREBA and CREBa, of
which the rat and human homologues have been
previously identified. Both encode proteins with CREbinding
activity and identical transactivation potential.
The additional CREB mRNA isoforms potentially encode
CREB related proteins. From the structural organization
of the mouse CREB gene we conclude that the multiple
transcripts are generated by alternative splicing. Furthermore
we show that specific CREB mRNA isoforms are
expressed at a high level in the adult testis. Expression
of these isoforms is induced after commencement of
spermatogenesis. In situ hybridization suggests that this
expression occurs predominantly in the primary spermatocytes.
Comparison of the CREB gene with the recently
isolated CREM (cAMP responsive element modulator)
cDNAs illustrates that the two genes have arisen by gene
duplication and have diverged to encode transcriptional
activators and repressors of the cAMP signal transduction
pathway
Studies on the Morphogenesis of Murine Cytomegalovirus
Two modes of assembly of murine cytomegalovirus (MCMV) were observed in cultured mouse embryo fïbroblasts, generating two morphologically different types of viral particles: monocapsid virions and multicapsid virions. The assembly of nucleocapsids appeared to be the same for both types of morphogenesis. Three successive stages of intranuclear capsid formation could be distinguished: capsids with electron-lucent cores, coreless capsids, and capsids with dense cores. Some of the capsids were enveloped at the inner nuclear membrane to form monocapsid virions, which were first detectable in the perinuclear cisterna. Other capsids left the nucleus via nuclear pores and usually entered cytoplasmic capsid aggregates that received an envelope by budding into extended cytoplasmic vacuoles, thereby forming multicapsid virions. Since the formation of multicapsid virions is not restricted to cell culture conditions and also occurs in vivo in immunosuppressed mice, multicapsid virions may play a role in the pathogenesis of cytomegalovirus infection
Host immune response to cytomegalovirus
To confirm that immediate-early (IE) genes of murine cytomegalovirus (MCMV) give rise to antigens recognized by specific cytolytic T lymphocytes (CTL), a 10.8-kilobase fragment of MCMV DNA which is abundantly transcribed at IE times was transfected into L cells expressing the Ld class I major histocompatibility glycoprotein. The viral genome fragment contains sequences of the three IE transcription units of MCMV: ie1, ie2, and ie3. In the transfected cell lines, only the predominant 2.75-kilobase transcript of ie1 and its translation product pp89 could be detected. The transfectants were analyzed for membrane expression of an IE antigen by employing clone IE1, an IE-specific CTL clone, as the probe. Only cells that expressed both the MCMV IE gene(s) and the Ld gene were recognized by the CTL clone
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